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written by Jennifer Field, Expedition 395 Onboard Outreach Officer

Expedition 395 scientists gather in Bremen for the sample party.
Expedition 395 scientists gather in Bremen for the sample party.

Five months after the return of Expedition 395, the science team has been reunited with a common goal: collecting approximately 19,000 samples from the cores taken during 395 and 395C. This reunion is delightfully termed a “sample party”. Instead of boutique cocktails and canapés, we have gathered in Bremen, Germany to extract pieces of the cores at precise intervals and locations based on the needs of the scientists. Sample requests were made months ago via a computer program which turned the scientists’ parameters into actual measurements on specific cores. It also recorded the needed volume of the sample and what type of method would be used to extract and package it. The program then created labels and QR codes for every sample. The laboratory technicians then put the labels on bags and organized the bags into batches based on the core. The cores were removed on large trolleys from the refrigerated storage unit and scientists pulled the appropriate core based on the sample bags. Scientists took every sample requested from a core regardless of whether it was for them or not.

Dr. Sid Hemming grins as she holds up a box of bagged-up samples.
Dr. Sidney Hemming

Depending on the research objectives of the scientist, the samples varied in volume and collection method. Dr. Sidney Hemming has four main projects. One of these is to fully explore the benthic stratigraphy from 0-2.5 million years ago; while other scientists from the party are focusing on older samples, Dr. Hemming would like to be able to explain the apparent difference in the rates of accumulation during this time period. To do this, she had samples of 20 ccs taken roughly every four centimeters down the length of the cores during this time period. Dr. Hemming will also try to discover whether or not there is geochemical evidence in the sediments that mark the initiation of glaciation. Another objective of hers is to try to determine what the sand layers that were found in the cores from the east coast of Greenland (site 1602) can indicate about the history of the Greenland margin from the most recent glacial period to the Oligocene (30 my). This may include erosion history, climate changes, previous glaciation, and tectonic changes from the rifting which originally formed the North Atlantic basin. One last interest of hers is to study Glauconite. Glauconite is a mineral found in ocean sediments typically described as being formed in shallow marine environments. Dr. Hemming is unsure of the veracity of this assumption, as the distinct mineral is often found in deep ocean sediments. She hopes to clear up some of the mystery.

Dr. Tom Dunkley-Jones smiles as he hoists a heavy-looking bag of samples.
Dr. Tom Dunkley-Jones

Dr. Tom Dunkley-Jones is researching biomarkers, in the form of alkenones, left by microscopic organisms called coccolithophores. Due to the processing of these samples, they must be protected from the soft plastic sample baggies, which give off similar chemicals during processing. These 20 – 30 cc samples were wrapped in foil before being put into the baggies. Through analyzing these samples, Dr. Dunkley-Jones hopes to look at the long term temperature change in the North Atlantic. Coccolithophores create alkenones (a type of fat) of different saturations depending on the temperature of the water that they are living in. By analyzing the saturation of the alkenones found in the fossilized organisms, a corresponding temperature can be inferred. This information is critical as current climate models are tested on the Pliocene climate and the initial indication is that they have not accurately predicted the ocean temperature. The models have predicted a cooler temperature than what has been found using this proxy data. The data from Expeditions 395 & 395C will add to the strength of the existing data for this time period.

The analysis of samples from these two expeditions will be ongoing for years to come and hopefully will lead to exciting discoveries for the 395 scientists and their colleagues.

It’s more than a party!

Ocean Acidification

Color Science and Ocean Cores

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Color Theory

Look at this core below (figure 1) and describe the colors and values you see.

Fig. 1) A small section of core: 401-U1611B-41R-2W from expedition 401

Some dark gray stripes, some light gray stripes, maybe some yellowish tones in the lightest stripes. Congratulations! You are applying color theory. Color theory is about describing the behavior of colors, such as mixing, color contrast, and color harmony. How colors look together and how they’re made is the basics of color theory application. It is often used by painters, but color theory is not just applicable for artists. It is necessary for the scientific world, including analysis of the ocean floor. Color theory is used as an aid for the functional applications of color as a science. To practice color science we need to first understand the international standards and practices for imaging.

In color science, we use CIELAB which stands for Commission International de l’Eclairage, or the International Commission on Illumination. They provide the recommendations for lighting, vision, color, and imaging. L*a*b* (pronounced “L star”, “a star”, and “b star”) stands for the coordinates that define a color numerically. The a* and b* signals relate to color, or chromaticity. A is related to redness or greenness. This means that a positive “a*” value (+a*) is more red, and a negative “a*” value (-a*) is more green. B is related to yellowness or blueness, so +b* is more yellow, and -b* is more blue. The values of a* and b* range from -128 to 128. The L* is the lightness channel and represents a value (black to white). L* is on a scale from 0-100, 0 being the whitest white we perceive, and 100 being the blackest black. The color of something can be found in this represented 3-axis model (figure 2).

Fig. 2) model of the CIELAB color space using 3-axis

CIELAB is designed to approximate human vision and is great for perceiving small differences in color. Unlike RGB or CMYK, the colors CIELAB defines are not defined by a monitor or printer, but instead relate to the CIE standard observer. The standard observer is an averaging of the results of color matching experiments under that particular laboratory’s conditions to create a set base value for future reflectance recordings. For ocean coring, machines like the Section Half Multisensor Logger (SHMSL) use the CIELAB system for imaging cores.

The SHMSL

Fig 3.) photo of the Section Half Multisensor Logger on the JOIDES Resolution scanning an ocean core.

The SHMSL measures two things, spectral reflectance and magnetic susceptibility. These are used to create core descriptions. Since the SHMSL uses CIELAB, it requires a standard observer to set the “base” values. To set the standard observer, the SHMSL has a color reflectance control set (figure 4). The reflectance control set is similar to the ColorChecker used in professional photography (figure 5). These color patches have a known spectral reflectance value and are designed to mimic the values of natural objects, or in this case potential sediment and hard rock colors. The SHMSL is calibrated using this control set and a white standard. It then uses this recorded reflectance value to adjust future values.

Fig. 4) A photo of the SHMSL color reflectance control set (left). Fig. 5) A photo of the Macbeth ColorChecker commonly used in photography (right).

Once calibrated and properly set up, the SHMSL is ready to read a core! Below is a finished reading of a core (figure 6). The three graphs at the bottom show the L*, a*, and b* values along the length of the core.

Fig. 6.1) Main IMS- SHMSL Data Acquisition Display (top). Fig 6.2) A zoomed in photo of the Main IMS- SHMSL Data Acquisition Display focusing only on the L*a*b* graph (bottom). 

The numbers at the bottom of each L*, a*, and b* graphs match with the length of the core in cm. For example, at 20cm this reading shows that the core had a L* value above 80, an a* value around -30, and a b* value of around 47. This means the color was lighter in value, more green than red and more yellow than blue. A color with these values looks roughly like this (figure 7):

Fig. 7) A photo of a pale, yellow-greenish color.

Machines like the SHMSL are important for identifying colors on ocean cores. As we humans age, the differences in color vision grow wider due to the yellowing of our lens over time. A person in their 50s will see colors in a more yellow tint than someone in their teens due to aging. The SHMSL sets a standard for the lighting and imaging in the laboratory, narrowing the divide to provide the most accurate reading of color on the core possible.

Applying to the core

So now we know how to read the machine, but what does the color of an ocean core actually tell us? Color differences are used to quantify how an object’s color can change over time from light exposure, heat, and humidity. In the case of ocean cores, “spectral data can be used to estimate the abundances of certain compounds,” (TAMU). This means, the light values of a core may tell us about potential organic content. For example, green cores may be an indication of glauconite (depending on location and geological time) which could indicate an ancient shallow marine environment. Look back at figure one. Based on what we know of this area of the ocean floor, this type of color contrast and coloration is a clear example of a dolomotisation sequence (the formation of dolomite). Colors are powerful tools used for studying our oceans, and our oceans are full of colorful knowledge waiting for those with eyes to see it.

Sources:

  1. Berns, R. S. (2016). Color science and the visual arts a guide for conservators, curators, and the curious. Los Angeles Getty Conservation Institute.
  1. TAMU. (2026). GCR Section Half Multisensor Core Logger (SHMSL) User Guide. Atlassian.net; Texas A&M University. https://tamu-eas.atlassian.net/wiki/spaces/LMUG/pages/7341017839/SHMSL+User+Guide. Updated 06 March 2026
  2. Erick Bravo, Imaging Specialist for X401 aboard the JOIDES Resolution. Accessed 28 June 2026.
  3. Ly, Bao & Dyer, Ethan & Feig, Jessica & Chien, Anna & Bino, Sandra. (2020). Research Techniques Made Simple: Cutaneous Colorimetry: A Reliable Technique for Objective Skin Color Measurement. The Journal of investigative dermatology. 140. 3-12.e1. 10.1016/j.jid.2019.11.003.
  4. Macbeth ColorChecker. (2026). Imatest.com. https://www.imatest.com/wp-content/uploads/2022/01/msccc_colorchecker_classic_front.jpg
  5. Banaś, W. (2024). Convert LAB to RGB – colordesigner.io. Colordesigner.io. https://colordesigner.io/convert/labtorgb

Image sources:

Figure 1: Source 3

Figure 2: Source 4

Figure 3-4,6: Source 2

Figure 5: Source 5

Figure 7: Source 6

Written by OCA 2026 Mentor, Kellan Moss

Color Science and Ocean Cores

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Ocean Acidification

No Cruise Without a CTD

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By Naomi Krauzig (GEOMAR)

As the research vessel METEOR heads north toward Germany, the CTD Lab has become quiet.

For the past four weeks, the CTD rosette (named after the three core variables it measures: conductivity, temperature, and depth) has been one of the busiest instruments on board. Day and night, it disappeared beneath the waves and returned with information about the entire water column.

Now the final station has been completed and the CTD rosette has been stored away for the last time. It feels like the right moment to reflect on a tool that has accompanied generations of oceanographers -and on a ship that has done the same.

Introduced in the 1970s, Conductivity-Temperature-Depth (CTD) systems revolutionized ocean observation by providing continuous measurements throughout the water column. When METEOR III entered service in 1986, the CTD was already the workhorse of physical oceanography. In the 1990s, it gained a trusted companion: the Lowered Acoustic Doppler Current Profiler (LADCP), capable of measuring ocean currents from the surface to the seafloor.

Figure 1: One of the very first CTD casts aboard the METEOR III during M5 in late 1987 (Screenshot from a video by Bernd Ueberschaer).

Aboard METEOR, the CTD rosette now also carries a suite of additional sensors measuring oxygen, chlorophyll, turbidity, photosynthetically active radiation, nitrate, and even particles and plankton through an Underwater Vision Profiler. At the same time, its Niskin bottles collect seawater samples for analyses of oxygen, nutrients, salinity, and other properties, providing a detailed picture of the water column.

During M219, this classic CTD/LADCP system helped us reveal some of the hidden “highways” of the tropical Atlantic. Along the 11°S section off Brazil, a key location for monitoring the Atlantic Meridional Overturning Circulation, CTD measurements identified distinct water masses through their temperature, salinity, and oxygen signatures. At the same time, the LADCPs captured the currents carrying them: the warm, northward-flowing North Brazil Undercurrent in the upper ocean and the colder, southward-flowing Deep Western Boundary Current nearly two kilometers below.

Figure 2. One of the many stories revealed by a CTD section: dissolved oxygen along 11°S off Brazil, highlighting the layered structure of the tropical Atlantic, including an oxygen minimum (dark blue) and the indicated ventilation pathways of different water masses.

Further north, along 23°W, we crossed the equator and encountered one of the strongest subsurface currents in the world ocean: the Equatorial Undercurrent. Hidden just beneath the surface, this powerful eastward-flowing jet transports enormous amounts of water, heat, oxygen, nutrients, and carbon across the Atlantic: roughly one hundred times the discharge of the Amazon River!

Figure 3. Velocity structure observed along the 23°W transect crossing the equator. The LADCP measurements reveal the Equatorial Undercurrent, a strong eastward-flowing current centered around 50-150 m depth (dark red).

While these observations allow us to investigate water masses, currents, and the circulation of the tropical Atlantic, they also carried an additional meaning for many on board.

For four decades, CTD rosettes have been lowered from the deck of METEOR III in every ocean of the world, helping scientists understand complex ocean processes, monitor changes, and train generations of oceanographers. During more than 11,940 days at sea, thousands of stations have been completed from her deck. Countless students, technicians, crew members, and scientists have contributed to these observations, and many have built their careers around the data collected aboard this vessel.

To take part in the final cruise -and the final CTD cast- of METEOR III was a privilege. Over the course of this voyage, it became impossible not to notice the connection many people have with this vessel. For some, METEOR has been a second home for years. Colleagues became lifelong friends, sometimes even family, and countless memories were made during deployments, watches, and transits at sea. The research vessel, the discoveries, and even the familiar CTD rosette hold a special place in many hearts.

As we pack the last equipment and the laboratories become emptier, it is difficult not to wonder what comes next. METEOR IV will soon continue the tradition, equipped with new capabilities and ready to tackle the scientific questions of the coming decades. New technologies will undoubtedly expand how we observe the ocean, yet some traditions are likely to endure.

Figure 4. The rosette during the final CTD cast of M219. Besides the CTD, it carries Niskin bottles for seawater sampling, a suite of biogeochemical sensors, and the yellow-and-black LADCPs that measure ocean currents throughout the water column. This deployment marked the final CTD station of the cruise and the last CTD cast from RV METEOR III after forty years of service.

https://www.oceanblogs.org/m219/2026/06/27/no-cruise-without-a-ctd/

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Ocean Acidification

Counting Snowflakes in the Darkness of the Deep Ocean

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By Joelle Habib (Laboratoire d’Océanographie Villefranche)

When I was a kid, I wanted to be a photographer. I still do, actually. But somewhere along the way, science intervened, and it gave me something I never expected: the chance to be an underwater photographer. Not the National Geographic kind who chases polar bears or waits weeks for a penguin to do something interesting. My subjects are smaller. Much, much smaller. I get to photograph the invisible life of the deep ocean, the tiny animals and sinking particles that most people never know exist. And the camera I use to do it descends to 6000 meters below the surface.

This instrument is called the UVP, or Underwater Vision Profiler. On this cruise, we deployed a UVP6 attached to the CTD rosette, profiling down to 4000 meters depth. The instrument activates automatically once its pressure sensor detects it is moving downward, takes up to 20 pictures per second all the way to the bottom of the cast. But before we talk about what the UVP gives us and why it matters, we need to talk about what it actually photographs: zooplankton and particles.

If you have ever watched SpongeBob SquarePants, you already know a zooplankton. Sheldon J. Plankton, the tiny villain who is perpetually trying to steal the Krabby Patty formula, is one. And funnily enough, the most abundant zooplankton across all the world’s oceans, is indeed this small crustacean: the copepod.

Here is the basic idea: a plankton is any organism that drifts with the ocean currents rather than swimming against them. If it photosynthesizes like a plant and contains chlorophyll pigments, it is a phytoplankton. If it is an animal, it is a zooplankton. A jellyfish is a zooplankton, just a very large one. Zooplankton graze on phytoplankton, on each other, and on anything small enough to eat. Now for the process that connects all of this to climate, to carbon, and to why we are out here on a research vessel in the middle of the equatorial Atlantic: the biological pump.

The biological pump is the ocean’s mechanism for pulling carbon out of the atmosphere and locking it away in the deep sea. Here is how it works: phytoplankton at the surface absorb CO₂ from the atmosphere and convert it into organic matter through photosynthesis. When they die, or when zooplankton eat them, defecate, excrete, and die themselves, all of that organic carbon does not simply disappear. It becomes marine snow! Yes, it snows in the ocean!!! Marine snow consists of a continuous rain of particles, aggregates, fecal pellets, shed exoskeletons, … Every flake of marine snow is a fragment of life that once existed at the surface, now on a one-way journey into the deep. This is the gravitational pump, one of the most important carbon sinks on Earth, and it is one of the pumps that the UVP was built to observe.

Marine snow seen by PELAGIOS (Pelagic In situ Observation System) in the Tropical Atlantic; 23°W; 100 m depth. Photo Credit: Henk-Jan Hoving

So why image and count particles rather than simply collecting water samples or relying on sediment traps? Because the abundance and size distribution of marine particles are two of the major factors controlling biological carbon sequestration in the ocean, and traditional methods cannot capture them at high resolution throughout the water column. Vertical profiles of particle images can reveal the processes that determine particle size, type, and distribution, and combined with information on carbon content and sinking velocity, they provide high-resolution information on how the biological pump operates at depth. The UVP allows for the remote collection of large datasets on particle abundances and their size distributions, enabling much higher spatial and temporal resolution than traditional methods. But particles are only one part of the story, the UVP also tracks zooplankton and their daily migrations: every night, zooplankton rise from the deep to feed near the surface, then sink back down before dawn, actively carrying carbon into the deep ocean in their own bodies. Without imaging tools like the UVP, this active carbon flux is nearly impossible to quantify.

A mosaic of unprocessed UVP images of particles and zooplankton from the M219 cruise

Each image you see here was taken in complete darkness, somewhere between the ocean surface and 4000 meters below. The UVP6 illuminates a tiny volume of water, with a single red flashing light, capturing only the particles and organisms that happen to drift through that small window at that exact moment.

The instrument captures everything larger than 100 micrometers, roughly the width of a human hair. In the images you will see two types of things: fuzzy, irregular blobs of varying sizes: Marine snow aggregates. And more defined, structured shapes, sometimes with appendages, antennae, or transparent shells. Those are the zooplankton.

Every image is a small portrait of a world that already existed long before we had the tools to see it. I am so lucky I am able to see parts of that hidden life in this lifetime.

Counting Snowflakes in the Darkness of the Deep Ocean

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