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いらっしゃいませ – welcome!

Greetings

GAME is back in Japan! Once again, an international two-person team, made up of a German and a Japanese student, is based at the Akkeshi Marine Station on Hokkaido, Japan, to contribute to this year’s pioneering research on the effect of artificial light at night on marine macroalgae.

Team Japan 2024: Hikari and Martin at the Akkeshi Marine Station. Photo: Team Japan 2024.

GAME

GAME projects have constituted an important part in global oceanic research for well over two decades. Sophisticated experimental set ups, which are replicated over a broad range of climatic and geographic areas around the globe do not only provide valuable scientific data for single systems, but also enable a global comparison of the results between latitudes, climate zones and biogeographic regions. In times in which we face universal environmental issues like climate change and the loss of biodiversity, it is becoming increasingly important to conduct experiments on a bigger scale.

ALAN and macroalgae

Just as in the last three years, this year’s GAME teams will investigate an anthropogenic influence on marine ecosystems that so far has not received the attention which it deserves – light pollution. Although, it is not a field that is of interest for many people, including even marine biologists and oceanographers, light pollution is by now regarded as one of the fastest growing human impacts on coastal ecosystems of the last twenty years.

Almost unnoticed, artificial light at night (ALAN) became a constant companion of modern life and this also applies to coastlines, of which some are among the most densely populated regions on earth. In these areas, seeing the milky way when walking alongside a beach has become practically impossible. Direct illumination by coastal infrastructures like houses, streetlights, and harbors as well as indirect enlightening of the coast through the so-called “skyglow”, i.e. artificial light reflected by clouds, have deprived us of this beautiful experience. However, although, we can directly experience the consequences of this change in night-time lightscapes, so far little is known about the consequences for underwater life. This is particularly true for the potential influence of ALAN on macroalgae, which are very important marine photoautotroph organisms. Almost no research has so far been conducted on this topic. GAME 2024 investigates the impact of ALAN on different species of macroalgae and its possible interplay with another important stressors for aquatic plants – grazing.

Macroalgae are aquatic photoautotrophs that can form extensive stands in shallow waters. The large-growing brown macroalgae are also called kelps. Martin in front of the Akkeshi Marine Station with an example of a kelp species: Costaria costata. Photo: Team Japan 2024

Why could artificial light at night affect macroalgae? As photoautotrophic organisms, just like terrestrial vascular plants, they need periodical light-dark rhythms to maintain their growth and vitality. The latter ensures the stability of macroalgae populations, and this not only relevant for the integrity of coastal ecosystems. Macroalgae provide multiple important ecosystem services to us such as coastal protection, carbon fixation and food supply. Therefore, it is crucial to understand how nightly illumination could impact the performance of these organisms.

Akkeshi

Akkeshi-chō (Akkeshi town) is a perfect locality regarding ALAN research as we can find areas with varying levels of light pollution in the close surroundings. Areas heavily lit throughout the night like the Akkeshi harbor can be found as well as the Aikappu cape, where basically no artificial light at night can be measured. Especially in this project year, with its focus laying on macroalgae, Japan’s northern coast constitutes a perfect place for this kind of research. The cold temperate climate and the nutrient rich waters support a huge variety of macroalgae, which are also important for the economy of the region as well as for the above mentioned ecosystem services.

Aikappu Cape is a place that is free of light pollution. Photo: Team Japan 2024.

But also besides being a fantastic place for our research, this area has a lot to offer. The Akkeshi Sakura (Cherry blossom) & Oyster Festival is just around the corner of the marine station, and it is supposed to be one of the highlights of the year! The oyster culture can be experienced here at every corner. There are multiple izakaya in Akkeshi, which serve delicious oysters – many of them are still run by the local oyster farmers themselves.

During longer trips around Hokkaido you can visit the world-famous Shiretoko National Park or the beautiful cities of Hakodate and Sapporo. Furthermore, there are multiple beautiful lakes and a variety of natural shitsugen (wetlands) worth visiting

Akkeshi Marine Station

The Akkeshi Marine Station is an external research unit of the University of Hokkaido in Sapporo located at the east coast of Japan’s northernmost main island. It has been a valuable site for applied research to the GAME projects for many years. Apart from its exquisite location for macroalgae, it is an outstandingly well-equipped facility with a great team of fellow Japanese master and PhD students as well as renowned scientist in various field of marine research (seagrass, phytoplankton, marine mammals, microplastic, peracarid crustaceans, etc.).

View from the guest house of the Akkeshi Marine Station towards the laboratory. Photo: Team Japan 2024.
A seagras meadow near the Akkeshi Marine Station. Photo: Team Japan 2024.

The station lies within the Akkeshi-Kiritappu-Konbumori Quasi-National Park, where daytrips can lead you from the tidal flats of the Akkeshi-ko (Lake Akkeshi) and the oak and maple forests to the bamboo-covered scarps of the Namida-misaki cape (Cape of Tears – but don’t worry, it will be tears of joy), where herds of Sika deer are bearingly grazing. With a little bit of luck, you can also see the local rakko (sea otters) from there. Outdoorsiness will therefore definitely pay off… 😉

Martin

My name is Martin (29) and I represent the “German” part of this year’s GAME team in Akkeshi, Japan. I was born and raised in the very west of the Austrian Alps and started my biological career more or less far away from the ocean in Styria, the so-called “Austrian Tuscany”. Through acquaintances with the GAME participants at the study site in Croatia back in 2021 I first got to know about this program and was immediately fascinated by it. Though back then I didn’t think that I will participate in it myself one day. When I started my master course in marine biology at the University of Rostock in northern Germany it became clear to me very soon that this is the kind of scientific consortium that I wanted to be a part of.

This is my first visit to Japan, and it has been very fascinating so far. Although it is still very cold – spring season seems to start very late around here – I was already able to experience some of the natural beauties in this area. The Bekambeushi-shitsugen is a Ramsar-registered wetland area around Akkeshi town and the second biggest in all of Japan. It has a unique waterfowl diversity (especially the famous red-crowned crane, Grus japonensis) and is supposed to be beautiful for kayak trips (let’s hope it will get warmer soon 😊).

Another great experience so far was the rocky shore just in front of the station with its countless tidepools. A huge variety of all kinds of organisms (macroalgae, crustaceans, echinoderms, molluscs, etc.) can be found there, which are vastly different and much bigger than what I am used to from the Baltic and North Sea. The local seagrass meadows grow up to two meters tall and the kelp forests (brown algae) can even reach five to six meters in length. The variety of occurring algae is also mindblowing. Altogether more than 200 macroalgae species can be found around this area, of which we choose some of the most dominant and important species to conduct our experiments with.

A short walk away from the station also lies the Akkeshi National History Museum, which our team’s supervisor, Masahiro Nakaoka, is the curator of and which is definitely worth a visit.

Hikari

Hi, I’m Hikari (22) and I am studying in the master program “Aquatic biology” at Hokkaido University. My hometown is far from any coastline, which made me longing to live near the sea and to study about the ocean for a long time. I visited the Akkeshi Marine Station for the first time for a practical training two years ago and I was completely captivated by the beautiful scenery. Therefore, I permanently relocated to Akkeshi last year. My motivation for this project is to obtain profound knowledge and gain as much experience on macroalgae research as possible.

Hikari and Martin. Photo: Team Japan 2024.

Site specific work

By now, we’re about to start the main experiments. In the beginning, we checked our material and devices and conducted some light measurements on different light sources, spectra and intensities. As my (Martin 😊) Japanese is not that fluent so far, I have encountered some minor communication problems with the in-house technicians (unfortunately they’re not so fluent in English), but with the help of Hikari we still managed to communicate our wishes and concerns. Thanks a lot at this point to the technicians, Hamano-san and Hide-san, for their great help! ありがとうございます – arigatou gozaimasu!

During the past weeks we worked on setting up our shelves, on which we will expose macroalgae from the nearby sea to different night time light regimes. The main tasks for us so far were the installation of the water flow-through system and the mounting of the LED lights in the laboratory. It was a lot of fuzzy work to get everything exactly at the spot we want it to be but in the end we managed to do so. Hopefully everything stays at its place for the next 5 months – fingers crossed… Besides the area, where we will conduct our experiments, the laboratory contains multiple other aquariums of all sorts and sizes where simultaneously other scientists and student are working on their experiments. The station and its aquarium room literally are a stone’s throw away from the intertidal area of Akkeshi Bay, which makes the collection and the transport of algae and grazers to the laboratory very fast and keeps the impact to the organisms to a minimum. 

Martin equipping a shelf in the laboratory with the LED strips for the main experiments. Photo: Team Japan 2024.

After having covered the whole shelf with light impermeable foil, we started to set up the scene for our pilot studies, during which we gained additional knowledge about the interaction of the algae and grazer species we work with. To gain the most valuable information about the effects of ALAN, we decided to work with the most abundant and important species of the local coastal ecosystem. Our choice for the algal target species fell on Saccharina japonica (a local brown algae species of kombu, which is also very important economically), Chondrus yendoi (a very abundant red algae, which is very important as a food resource for most of the intertidal species) and Fucus distichus (a habitat building brown algae crucial for the vitality of the coastal area). To feed on our algae we decided to work with Idotea ochotensis, a regional species of marine isopod, which is inconspicuous to the eye at first, but due to its abundance and voracity plays an essential role in the coastal food web and the remineralization process of organic material. For obtaining more detailed information on the interaction of these species with each other, we will assess the consumption rates of the isopods on our algae as well as if they prefer to graze during the day or during the night.

A beached specimen of the brown macroalga Saccharina japonica. Photo: Team Japan 2024.
This is the grazer species we are using for the main experiments. The marine isopod Idotea ochotensis. Photo: Team Japan 2024.

In the next days, after having accomplished several test runs on the experimental set up as well as having practiced to conduct measurements with the laboratory equipment, we will start our main experiments.

お疲れ様です – thanks for your hard work!

Enlightenment in Japan – how artificial light at night influences local kelp forests.

Ocean Acidification

Color Science and Ocean Cores

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Color Theory

Look at this core below (figure 1) and describe the colors and values you see.

Fig. 1) A small section of core: 401-U1611B-41R-2W from expedition 401

Some dark gray stripes, some light gray stripes, maybe some yellowish tones in the lightest stripes. Congratulations! You are applying color theory. Color theory is about describing the behavior of colors, such as mixing, color contrast, and color harmony. How colors look together and how they’re made is the basics of color theory application. It is often used by painters, but color theory is not just applicable for artists. It is necessary for the scientific world, including analysis of the ocean floor. Color theory is used as an aid for the functional applications of color as a science. To practice color science we need to first understand the international standards and practices for imaging.

In color science, we use CIELAB which stands for Commission International de l’Eclairage, or the International Commission on Illumination. They provide the recommendations for lighting, vision, color, and imaging. L*a*b* (pronounced “L star”, “a star”, and “b star”) stands for the coordinates that define a color numerically. The a* and b* signals relate to color, or chromaticity. A is related to redness or greenness. This means that a positive “a*” value (+a*) is more red, and a negative “a*” value (-a*) is more green. B is related to yellowness or blueness, so +b* is more yellow, and -b* is more blue. The values of a* and b* range from -128 to 128. The L* is the lightness channel and represents a value (black to white). L* is on a scale from 0-100, 0 being the whitest white we perceive, and 100 being the blackest black. The color of something can be found in this represented 3-axis model (figure 2).

Fig. 2) model of the CIELAB color space using 3-axis

CIELAB is designed to approximate human vision and is great for perceiving small differences in color. Unlike RGB or CMYK, the colors CIELAB defines are not defined by a monitor or printer, but instead relate to the CIE standard observer. The standard observer is an averaging of the results of color matching experiments under that particular laboratory’s conditions to create a set base value for future reflectance recordings. For ocean coring, machines like the Section Half Multisensor Logger (SHMSL) use the CIELAB system for imaging cores.

The SHMSL

Fig 3.) photo of the Section Half Multisensor Logger on the JOIDES Resolution scanning an ocean core.

The SHMSL measures two things, spectral reflectance and magnetic susceptibility. These are used to create core descriptions. Since the SHMSL uses CIELAB, it requires a standard observer to set the “base” values. To set the standard observer, the SHMSL has a color reflectance control set (figure 4). The reflectance control set is similar to the ColorChecker used in professional photography (figure 5). These color patches have a known spectral reflectance value and are designed to mimic the values of natural objects, or in this case potential sediment and hard rock colors. The SHMSL is calibrated using this control set and a white standard. It then uses this recorded reflectance value to adjust future values.

Fig. 4) A photo of the SHMSL color reflectance control set (left). Fig. 5) A photo of the Macbeth ColorChecker commonly used in photography (right).

Once calibrated and properly set up, the SHMSL is ready to read a core! Below is a finished reading of a core (figure 6). The three graphs at the bottom show the L*, a*, and b* values along the length of the core.

Fig. 6.1) Main IMS- SHMSL Data Acquisition Display (top). Fig 6.2) A zoomed in photo of the Main IMS- SHMSL Data Acquisition Display focusing only on the L*a*b* graph (bottom). 

The numbers at the bottom of each L*, a*, and b* graphs match with the length of the core in cm. For example, at 20cm this reading shows that the core had a L* value above 80, an a* value around -30, and a b* value of around 47. This means the color was lighter in value, more green than red and more yellow than blue. A color with these values looks roughly like this (figure 7):

Fig. 7) A photo of a pale, yellow-greenish color.

Machines like the SHMSL are important for identifying colors on ocean cores. As we humans age, the differences in color vision grow wider due to the yellowing of our lens over time. A person in their 50s will see colors in a more yellow tint than someone in their teens due to aging. The SHMSL sets a standard for the lighting and imaging in the laboratory, narrowing the divide to provide the most accurate reading of color on the core possible.

Applying to the core

So now we know how to read the machine, but what does the color of an ocean core actually tell us? Color differences are used to quantify how an object’s color can change over time from light exposure, heat, and humidity. In the case of ocean cores, “spectral data can be used to estimate the abundances of certain compounds,” (TAMU). This means, the light values of a core may tell us about potential organic content. For example, green cores may be an indication of glauconite (depending on location and geological time) which could indicate an ancient shallow marine environment. Look back at figure one. Based on what we know of this area of the ocean floor, this type of color contrast and coloration is a clear example of a dolomotisation sequence (the formation of dolomite). Colors are powerful tools used for studying our oceans, and our oceans are full of colorful knowledge waiting for those with eyes to see it.

Sources:

  1. Berns, R. S. (2016). Color science and the visual arts a guide for conservators, curators, and the curious. Los Angeles Getty Conservation Institute.
  1. TAMU. (2026). GCR Section Half Multisensor Core Logger (SHMSL) User Guide. Atlassian.net; Texas A&M University. https://tamu-eas.atlassian.net/wiki/spaces/LMUG/pages/7341017839/SHMSL+User+Guide. Updated 06 March 2026
  2. Erick Bravo, Imaging Specialist for X401 aboard the JOIDES Resolution. Accessed 28 June 2026.
  3. Ly, Bao & Dyer, Ethan & Feig, Jessica & Chien, Anna & Bino, Sandra. (2020). Research Techniques Made Simple: Cutaneous Colorimetry: A Reliable Technique for Objective Skin Color Measurement. The Journal of investigative dermatology. 140. 3-12.e1. 10.1016/j.jid.2019.11.003.
  4. Macbeth ColorChecker. (2026). Imatest.com. https://www.imatest.com/wp-content/uploads/2022/01/msccc_colorchecker_classic_front.jpg
  5. Banaś, W. (2024). Convert LAB to RGB – colordesigner.io. Colordesigner.io. https://colordesigner.io/convert/labtorgb

Image sources:

Figure 1: Source 3

Figure 2: Source 4

Figure 3-4,6: Source 2

Figure 5: Source 5

Figure 7: Source 6

Written by OCA 2026 Mentor, Kellan Moss

Color Science and Ocean Cores

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Ocean Acidification

No Cruise Without a CTD

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By Naomi Krauzig (GEOMAR)

As the research vessel METEOR heads north toward Germany, the CTD Lab has become quiet.

For the past four weeks, the CTD rosette (named after the three core variables it measures: conductivity, temperature, and depth) has been one of the busiest instruments on board. Day and night, it disappeared beneath the waves and returned with information about the entire water column.

Now the final station has been completed and the CTD rosette has been stored away for the last time. It feels like the right moment to reflect on a tool that has accompanied generations of oceanographers -and on a ship that has done the same.

Introduced in the 1970s, Conductivity-Temperature-Depth (CTD) systems revolutionized ocean observation by providing continuous measurements throughout the water column. When METEOR III entered service in 1986, the CTD was already the workhorse of physical oceanography. In the 1990s, it gained a trusted companion: the Lowered Acoustic Doppler Current Profiler (LADCP), capable of measuring ocean currents from the surface to the seafloor.

Figure 1: One of the very first CTD casts aboard the METEOR III during M5 in late 1987 (Screenshot from a video by Bernd Ueberschaer).

Aboard METEOR, the CTD rosette now also carries a suite of additional sensors measuring oxygen, chlorophyll, turbidity, photosynthetically active radiation, nitrate, and even particles and plankton through an Underwater Vision Profiler. At the same time, its Niskin bottles collect seawater samples for analyses of oxygen, nutrients, salinity, and other properties, providing a detailed picture of the water column.

During M219, this classic CTD/LADCP system helped us reveal some of the hidden “highways” of the tropical Atlantic. Along the 11°S section off Brazil, a key location for monitoring the Atlantic Meridional Overturning Circulation, CTD measurements identified distinct water masses through their temperature, salinity, and oxygen signatures. At the same time, the LADCPs captured the currents carrying them: the warm, northward-flowing North Brazil Undercurrent in the upper ocean and the colder, southward-flowing Deep Western Boundary Current nearly two kilometers below.

Figure 2. One of the many stories revealed by a CTD section: dissolved oxygen along 11°S off Brazil, highlighting the layered structure of the tropical Atlantic, including an oxygen minimum (dark blue) and the indicated ventilation pathways of different water masses.

Further north, along 23°W, we crossed the equator and encountered one of the strongest subsurface currents in the world ocean: the Equatorial Undercurrent. Hidden just beneath the surface, this powerful eastward-flowing jet transports enormous amounts of water, heat, oxygen, nutrients, and carbon across the Atlantic: roughly one hundred times the discharge of the Amazon River!

Figure 3. Velocity structure observed along the 23°W transect crossing the equator. The LADCP measurements reveal the Equatorial Undercurrent, a strong eastward-flowing current centered around 50-150 m depth (dark red).

While these observations allow us to investigate water masses, currents, and the circulation of the tropical Atlantic, they also carried an additional meaning for many on board.

For four decades, CTD rosettes have been lowered from the deck of METEOR III in every ocean of the world, helping scientists understand complex ocean processes, monitor changes, and train generations of oceanographers. During more than 11,940 days at sea, thousands of stations have been completed from her deck. Countless students, technicians, crew members, and scientists have contributed to these observations, and many have built their careers around the data collected aboard this vessel.

To take part in the final cruise -and the final CTD cast- of METEOR III was a privilege. Over the course of this voyage, it became impossible not to notice the connection many people have with this vessel. For some, METEOR has been a second home for years. Colleagues became lifelong friends, sometimes even family, and countless memories were made during deployments, watches, and transits at sea. The research vessel, the discoveries, and even the familiar CTD rosette hold a special place in many hearts.

As we pack the last equipment and the laboratories become emptier, it is difficult not to wonder what comes next. METEOR IV will soon continue the tradition, equipped with new capabilities and ready to tackle the scientific questions of the coming decades. New technologies will undoubtedly expand how we observe the ocean, yet some traditions are likely to endure.

Figure 4. The rosette during the final CTD cast of M219. Besides the CTD, it carries Niskin bottles for seawater sampling, a suite of biogeochemical sensors, and the yellow-and-black LADCPs that measure ocean currents throughout the water column. This deployment marked the final CTD station of the cruise and the last CTD cast from RV METEOR III after forty years of service.

https://www.oceanblogs.org/m219/2026/06/27/no-cruise-without-a-ctd/

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Ocean Acidification

Counting Snowflakes in the Darkness of the Deep Ocean

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By Joelle Habib (Laboratoire d’Océanographie Villefranche)

When I was a kid, I wanted to be a photographer. I still do, actually. But somewhere along the way, science intervened, and it gave me something I never expected: the chance to be an underwater photographer. Not the National Geographic kind who chases polar bears or waits weeks for a penguin to do something interesting. My subjects are smaller. Much, much smaller. I get to photograph the invisible life of the deep ocean, the tiny animals and sinking particles that most people never know exist. And the camera I use to do it descends to 6000 meters below the surface.

This instrument is called the UVP, or Underwater Vision Profiler. On this cruise, we deployed a UVP6 attached to the CTD rosette, profiling down to 4000 meters depth. The instrument activates automatically once its pressure sensor detects it is moving downward, takes up to 20 pictures per second all the way to the bottom of the cast. But before we talk about what the UVP gives us and why it matters, we need to talk about what it actually photographs: zooplankton and particles.

If you have ever watched SpongeBob SquarePants, you already know a zooplankton. Sheldon J. Plankton, the tiny villain who is perpetually trying to steal the Krabby Patty formula, is one. And funnily enough, the most abundant zooplankton across all the world’s oceans, is indeed this small crustacean: the copepod.

Here is the basic idea: a plankton is any organism that drifts with the ocean currents rather than swimming against them. If it photosynthesizes like a plant and contains chlorophyll pigments, it is a phytoplankton. If it is an animal, it is a zooplankton. A jellyfish is a zooplankton, just a very large one. Zooplankton graze on phytoplankton, on each other, and on anything small enough to eat. Now for the process that connects all of this to climate, to carbon, and to why we are out here on a research vessel in the middle of the equatorial Atlantic: the biological pump.

The biological pump is the ocean’s mechanism for pulling carbon out of the atmosphere and locking it away in the deep sea. Here is how it works: phytoplankton at the surface absorb CO₂ from the atmosphere and convert it into organic matter through photosynthesis. When they die, or when zooplankton eat them, defecate, excrete, and die themselves, all of that organic carbon does not simply disappear. It becomes marine snow! Yes, it snows in the ocean!!! Marine snow consists of a continuous rain of particles, aggregates, fecal pellets, shed exoskeletons, … Every flake of marine snow is a fragment of life that once existed at the surface, now on a one-way journey into the deep. This is the gravitational pump, one of the most important carbon sinks on Earth, and it is one of the pumps that the UVP was built to observe.

Marine snow seen by PELAGIOS (Pelagic In situ Observation System) in the Tropical Atlantic; 23°W; 100 m depth. Photo Credit: Henk-Jan Hoving

So why image and count particles rather than simply collecting water samples or relying on sediment traps? Because the abundance and size distribution of marine particles are two of the major factors controlling biological carbon sequestration in the ocean, and traditional methods cannot capture them at high resolution throughout the water column. Vertical profiles of particle images can reveal the processes that determine particle size, type, and distribution, and combined with information on carbon content and sinking velocity, they provide high-resolution information on how the biological pump operates at depth. The UVP allows for the remote collection of large datasets on particle abundances and their size distributions, enabling much higher spatial and temporal resolution than traditional methods. But particles are only one part of the story, the UVP also tracks zooplankton and their daily migrations: every night, zooplankton rise from the deep to feed near the surface, then sink back down before dawn, actively carrying carbon into the deep ocean in their own bodies. Without imaging tools like the UVP, this active carbon flux is nearly impossible to quantify.

A mosaic of unprocessed UVP images of particles and zooplankton from the M219 cruise

Each image you see here was taken in complete darkness, somewhere between the ocean surface and 4000 meters below. The UVP6 illuminates a tiny volume of water, with a single red flashing light, capturing only the particles and organisms that happen to drift through that small window at that exact moment.

The instrument captures everything larger than 100 micrometers, roughly the width of a human hair. In the images you will see two types of things: fuzzy, irregular blobs of varying sizes: Marine snow aggregates. And more defined, structured shapes, sometimes with appendages, antennae, or transparent shells. Those are the zooplankton.

Every image is a small portrait of a world that already existed long before we had the tools to see it. I am so lucky I am able to see parts of that hidden life in this lifetime.

Counting Snowflakes in the Darkness of the Deep Ocean

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