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We explore the entire tree of life on the research vessel MARIA S. MERIAN: animals, algae, bacteria, and viruses. The cruise focuses on the food web and ocean biodiversity. However, some animals, especially hunters such as squid and whales, are too big, fast or camera-shy for our instruments. To detect these top predators, we search for and collect genetic traces, so to speak, working as zoological forensic scientists. Ocean animals constantly lose cells and DNA when swimming, breathing, eating and defecating, resulting in the release of genetic material. We collect and identify this so-called environmental DNA (eDNA for short) using a method specifically developed to analyse DNA traces collected from environmental samples. Specific DNA sections, such as ribosomal DNA or genes essential for energy production, are analysed and exist in all animals. The same or similar essential genes are used for bacteria or unicellular organisms. These genes are later amplified from the samples via PCR and sequenced via next-generation sequencing technologies. The results are later compared to genetic databases to discover which species occurred in the environmental sample.

So, while our colleagues filter water samples to study phytoplankton and microplankton, we filter water from the CTD in parallel and analyse the DNA later in Kiel in the GEOMAR laboratories. Based on the specific DNA traces, we can determine the presence of certain species, such as the deep-sea cephalopods Histioteuthis and Heteroteuthis. Water sampled every 100 m from the surface down to the seafloor can show us at what depths cephalopods occur and allows us to predict what species of prey predatory whales may encounter during their dives. For this, we work together with cetacean specialists. At the same time, we also analyse the biodiversity of other organisms in the samples, which we could then compare to predict the prey of the cephalopods. Ultimately, we can also compare our genetic results with the distribution and abundance of the diverse species of plankton and animals identified by the MSM126 team members, each of whom has their own taxonomic speciality.

Minutes after deployment of the deep-sea lander with bait in the black nets at 1500m depth, the first guests arrived. Photo: ROV team GEOMAR

We are also carrying out an innovative eDNA experiment on the deep seafloor during our expedition. For this, the deep-sea robot or ROV has deployed a deep-sea lander with bait on the seafloor to mimic the deposition of a carcass from the overlying water column. This carcass attracts and nourishes many organisms since deep-sea bottom organisms largely depend on food from the overlying water column. We returned to this site several times with the ROV robot to take sediment cores and water samples. These samples are immediately preserved on board for future eDNA analysis. The experiment will investigate 1) which species are attracted to the food deposition event and 2) to what extent the biodiversity and ecosystem of animals and bacteria change over time after the food deposition event.

Environmental genetics, the search for traces in the sea, is a modern addition that supports various classical approaches in marine research and contributes new information and perspectives. It is, therefore, also an essential part of MSM126 to combine research from different disciplines and gain new insights.

So-called push corers are used to collect sediment cores with the ROV robot. Photo: ROV team GEOMAR

Forensics in the sea

Ocean Acidification

Keeping the Record Alive: Long-Term Ocean Observations in the Tropical Atlantic

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By Naomi Krauzig (GEOMAR)

One of the most rewarding aspects of M219 has been contributing to the maintenance of the long-term GEOMAR mooring arrays that quietly monitor the tropical Atlantic year after year.

While CTD/LADCP casts and other shipboard measurements provide invaluable snapshots of the ocean, these anchored instruments provide something that cannot be obtained otherwise: continuous observations spanning minutes, days, seasons, years, and even decades. As an observational oceanographer, it is difficult not to appreciate the value of these datasets. They form the foundation for understanding ocean variability in regions that are critical for Atlantic climate variability and allow us to detect and quantify long-term changes that would otherwise remain hidden within the ocean’s natural variability.

Our first major operations took place off the Brazilian coast at 11°S, where the K1 to K4 moorings form part of a long-term observing system monitoring the western boundary current system and the Atlantic Meridional Overturning Circulation (AMOC). Within just a few days, the four deep-sea moorings were successfully recovered, assessed, serviced, and redeployed.

Every recovery felt a bit like opening a treasure chest. After spending a year or more beneath the ocean surface, these instruments returned carrying an invaluable record of currents, temperature, salinity, oxygen, and other key ocean properties. It was incredibly rewarding to see how well they had performed. Nearly all instruments operated successfully throughout the entire deployment period, delivering high-quality datasets with remarkably few gaps.

From Brazil, we continued north to the equator at 23°W, home to another key long-term mooring at exactly 0°N. Since 2006, this mooring has been monitoring the Equatorial Undercurrent and the deep equatorial circulation from the surface to nearly 4,000 m depth. Its successful recovery and redeployment mean that this unique 20-year time series will continue, helping us better understand how the tropical Atlantic influences climate, oxygen and nutrient transport, and marine ecosystems across the basin.

Our final mooring destination brought us to the Cape Verde Ocean Observatory (CVOO), one of the flagship long-term ocean observatories in the eastern tropical Atlantic. Here, physical, biogeochemical, and ecological observations come together to track how the ocean stores heat and carbon and how marine ecosystems respond to environmental change. Like the moorings at 11°S and the equator, the value of CVOO lies not in a single measurement, but in the continuity of the multi-decadal record.

For me, one of the most memorable aspects was seeing how many people contributed to the success of the mooring operations. Careful planning laid the foundation, while having a dedicated person keeping track of every step ensured that everything ran smoothly (kudos to Anna Christina Hans, aka Tina!). On deck, crew, technicians, and scientists worked together like a well-oiled machine, stepping in where needed and solving problems on the fly.

The teamwork extended all the way back home to GEOMAR. Thanks to Rebecca Hummels’ mooring toolbox, data from several instruments could already be processed and checked while parts of the moorings were still in the water, providing an early look at the quality of the observations. On top of that, mooring experts were available around the clock to provide information, advice, and troubleshooting whenever needed. I believe the high success rate of the recoveries and redeployments is a testament to the experience, teamwork, and dedication of everyone involved.

Redeployment of the K4 mooring showing the positive atmosphere and team spirit, despite long working hours and the demanding nature of the operations. Photo: Naomi Krauzig.

With the major milestone of the successful mooring work behind us, another exciting operation was still ahead. Waiting in Mindelo was a brand-new surface buoy, ready to begin its own contribution to these invaluable long-term observations. Stay tuned to learn more about that deployment in a future blog post.

Keeping the Record Alive: Long-Term Ocean Observations in the Tropical Atlantic

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Ocean Acidification

30 Days at Sea, 30 Ways to Make Potatoes

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By Joelle Habib (Laboratoire d’Océanographie Villefranche)

When you go on a scientific cruise, you always think about the instruments you’re going to deploy, the great data you’re going to acquire, or the experiments you’ll conduct. What you almost always forget is the small thing that isn’t actually small at all: food. And how are you going to eat it!

For those not familiar with scientific cruises: once you’re on board, most of your time goes to the science. You don’t really have time for food or food preparation. But there are always hidden heroes preparing your breakfast, lunch, and dinner, and, most importantly, the dessert for the dessert break. Today, instead of shedding light on the science, we’re going to talk about people, starting with the two chefs our lives basically depend on.

Rainer Götze and Peter Wernitz are the chefs of the last METEOR cruise. Rainer has been cooking on this ship for over 23 years, while Peter has been doing it for 13. Together they cook for 60 people on board, seamen and scientists alike. You’re probably wondering, like I was, how they pull it off. I had the chance to talk to them, and here are some of the ship’s secrets.

Let’s start with the planning. They don’t prepare the whole month’s menu before going on board, they plan it day by day. That said, a few dishes are practically law: fish on Tuesday and Friday, stew on Saturday (the stews are good, but it’s still my least favorite food day), and roasted meat on Sunday. Ice cream shows up for dessert on Sunday and Thursday lunches. And no matter the day, there’s always a vegetarian option on the table, nobody on board goes without something to eat.

So, all this cooking, but how many ingredients does it actually take? Let’s start with numbers. Every morning for breakfast there’s a choice of eggs (scrambled, boiled, fried…), pancakes, and more. So how many eggs are on this ship? For a one-month cruise, there are 3,000 eggs in storage, and the cooks go through around 90 of them a day. They also bake fresh bread every single day, about 3kg of flour goes into roughly 60 loaves. Coffee breaks happen all day, every day, there’s about 60kg of coffee on board. And since we’re on a German ship, and Germans do love their potatoes, there are 300kg of potatoes stored in a refrigerated, dark room so they don’t go bad.

You might be wondering why I’m talking so much about potatoes. Well, my dear reader, lunch has plenty of variety, but the one constant is potatoes. We’re on day 20 of the cruise, and I think we’ve worked through most of the varieties by now: fried, baked, soufflé, mashed, boiled and more still to come.

Another question I had was what happens if one of them gets sick. Rainer is a tough seaman who doesn’t get seasick anymore; Peter still does, occasionally. But either way, they’re always there, cooking through good conditions and bad. People generally love the food, though the chefs did tell me the one thing that never goes down well is old-school dishes like veal liver. (I can confirm.)

I think the message I’m trying to convey here is: a scientific cruise wouldn’t really be possible without Peter and Rainer. Science at sea is not only the science, but it’s also the work and effort of everyone on board. Especially the chefs!

Peter Wernitz and Rainer Götze in the METEOR’s kitchen. Photo: Joelle Habib

30 Days at Sea, 30 Ways to Make Potatoes

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Ocean Acidification

Where the sky meets the ocean

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By Leonie Jaeger (ICBM Oldenburg)

The ocean is the dominant climate regulator of our Earth. I am on board the RV Meteor to conduct measurements that helps us better understand the critical processes at the interface between the atmosphere and the ocean. The focus of these measurements is heat and freshwater fluxes, two key drivers that both influence and regulate Earth’s climate.

The ocean stores and transports vast amounts of heat across the whole globe. The exchange of heat between the atmosphere and the ocean is controlled by different surface heat fluxes. The sun emits shortwave radiation, which warms the surface ocean, though part of this radiation is reflected at the water surface. At the same time, the ocean emits longwave radiation towards the sky due to its temperature, some of which is reflected and absorbed by water vapor and clouds. To quantify these fluxes, I use radiometers: sets of upward- and downward-looking sensors that measure radiation coming from the sky and from the ocean. Specifically, pyranometers measure shortwave radiation, while pyrgeometers measure longwave radiation.

Radiometers to capture short- and longwave radiation coming from the sky and from the ocean to quantify radiative heat fluxes between the atmosphere and the ocean. Photo: Leonie Jaeger.

Over the open ocean, freshwater fluxes result from two processes: evaporation and precipitation. Approximately 80% of the global freshwater flux occurs over the ocean, underscoring the ocean’s dominance in the global water cycle and its influence on climate over land. In a warming climate, evaporation is expected to intensify as temperatures rise and the atmosphere’s capacity to hold moisture increases. That makes is very important to better understand these fluxes. However, high-quality measurements of precipitation and evaporation using remote techniques remain challenging. On this cruise, I am using a disdrometer, an instrument that measures rain in high resolution. It allows us to investigate not only the total amount of rain but also the velocity and size of individual raindrops, enabling a detailed characterization of rain events.  

A distant heavy rainfall event in the ITCZ over the central Atlantic Ocean. Photo: Leonie Jaeger.

Our cruise track crosses the Atlantic Ocean from South to North, passing the equator. This transect will provide a valuable dataset. Importantly, we will cross the Inter-Tropical Convergence Zone (ITCZ), a region near the equator characterized by heavy rain and thunderstorms. These storms originate from warm, moist air that rises continuously. As the air rises, it cools and condenses, forming thick clouds and intense precipitation. Because the ITCZ is driven by the convergence of trade winds from both hemispheres, it maintains persistent bands of convection. In this zone, these convective systems can trigger even more convection in the atmosphere driving the tropical climate. Together with warm surface temperatures, these high-energy processes can lead to the genesis of tropical cyclones. Thus, the atmosphere influences the ocean, and the ocean influences the atmosphere. Direct measurements at their interface are essential to better understand these processes shaping our climate. My responsibilities include installing and maintaining the measurements systems, as well as data validation and data storage. Maintaining sensors close to the ocean requires frequent cleaning, because sea spray leaves salt deposits everywhere, leading to corrosion. Together with ship-based measurements such as air temperature, wind speed and humidity, and oceanographic underway measurements including continuous observation of the water temperature, salinity, turbidity and chlorophyll, our data will provide a comprehensive dataset to study fresh and heat water fluxes between the ocean and the atmosphere.

Where the sky meets the ocean

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